open(FILE,"filename")||die"can not open the file: $!";
@filelist=<FILE>;

foreach $eachline (@filelist) {
        chomp $eachline;
}
close FILE;
@filelist=<FILE>;

open(FILE,"filename")||die"can not open the file: $!";
while (defined ($eachline =<FILE>)) {
     chomp $eachline;
         # do what u want here!
}
close FILE;