function readThis(){
var tempStr = "";
var filePath= document.all.upfile.value;
var oXL = new ActiveXObject("Excel.application");
var oWB = oXL.Workbooks.open(filePath);
oWB.worksheets(1).select();
var len = 100; // 要取到的多少條數據
var oSheet = oWB.ActiveSheet;
try{
for(var i=1;i<= len;i++){
tempStr+=("put(/""+oSheet.Cells(i,1).value+"/",/""+oSheet.Cells(i,2).value+"/");")+"<br>"; // 1和2分別代表第一列和第二列
}
}catch(e){
document.all.txtArea.innerHTML = tempStr;
}
document.all.txtArea.innerHTML = tempStr;
oXL.Quit();
CollectGarbage();
}

<input type="file" id="upfile" /><input type="button" onclick="readThis();" value="讀取">
<div id="txtArea" style="width:500px;height:500px;"></div>